tris glycine gradient gels (Bio-Rad)

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Bio-Rad tris glycine gradient gels
Tris Glycine Gradient Gels, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 21586 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gradient+tris+glycine+gels/Tris/pm42837722-269-10-13
Average 98 stars, based on 21586 article reviews

tris glycine gradient gels - by Bioz Stars, 2026-10

98/100 stars

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Staining:

Article Title: Targeting integrin beta 4 in diacetyl-induced anoikis of the airway epithelium
Article Snippet: Total protein concentrations were determined using a BCA assay kit (Thermo Scientific; Rockford, IL). .. Ten micrograms (μg) of total protein were resolved in stain-free, pre-cast 4–15% gradient Tris–Glycine gels (Bio-Rad; Hercules, CA), then transferred to 0.2 μm nitrocellulose membranes (Pall Corporation, NY). .. The membrane was stained with primary antibodies for keratin 5 (KRT5; 1:2000, Biolegend; San Diego, CA; Cat. # Poly19055), integrin beta 4 (ITGβ4, CD104; 1:1000, Invitrogen; Waltham, MA; Cat. # PA5-17172), eukaryotic initiation factor 2 alpha (eIF2α, Cell Signaling #9722, 1:1000), p-eIF2α (Cell Signaling #9721, 1:1000), and delta N isoform of transcription factor p63 (∆Np63; 1:1000, MilliporeSigma; Burlington, MA; Cat. #ABS552).

Article Title: Targeting integrin beta 4 in diacetyl-induced anoikis of the airway epithelium.
Article Snippet: Total protein concentrations were determined using a BCA assay kit (Thermo Scientific; Rockford, IL). .. Ten micrograms (μg) of total protein were resolved in stain-free, pre-cast 4–15% gradient Tris–Glycine gels (Bio-Rad; Hercules, CA), then transferred to 0.2 μm nitrocellulose membranes (Pall Corporation, NY). .. The membrane was stained with primary antibodies for keratin 5 (KRT5; 1:2000, Biolegend; San Diego, CA; Cat. # Poly19055), integrin beta 4 (ITGβ4, CD104; 1:1000, Invitrogen; Waltham, MA; Cat. # PA517172), eukaryotic initiation factor 2 alpha (eIF2α, Cell Signaling #9722, 1:1000), p-eIF2α (Cell Signaling #9721, 1:1000), and delta N isoform of transcription factor p63 (∆Np63; 1:1000, MilliporeSigma; Burlington, MA; Cat. #ABS552).

Incubation:

Article Title: Mapping the intracellular HMGB1 interactome and alterations induced by Toll-like receptor 4 activation
Article Snippet: .. 4 to 20% gradient Tris-Glycine gels (Bio-Rad) were loaded with equal amounts of protein (10–25 μg) and run at 110V for 1 h. Proteins were transferred to nitrocellulose blotting membranes (0.45 μm, GE Healthcare) at 100 V for 1 h. Membranes were blocked in 5% dry milk-TBST at RT for 1 h followed by incubation with HRP-conjugated primary antibodies or streptavidin-HRP (GE Healthcare, 1:10,000) at RT for 1 h. Incubation with non-conjugated primary antibodies was conducted at 4 °C o/n, followed by incubation with HRP-conjugated secondary antibodies (RT, 1 h). .. ECL-chemiluminescence was detected by ChemiDoc MP imaging system (Bio-Rad) or by exposing the membranes to X-Ray films (Amersham, GE Healthcare).

Nucleic Acid Electrophoresis:

Article Title: F26BP enables control of glycolysis rate independent of energy state
Article Snippet: Cellular protein was measured by PierceTM bicinchoninic acid (BCA) protein assay kit (Thermo Fisher Scientific #23227) to equalize protein quantities across samples. .. 10-20 μg of total cellular protein was analyzed by mixing with Laemmli buffer (BioRad #1610747, prepared in accordance to manufacturer’s instructions), separating by gel electrophoresis on 4–20% gradient Tris-Glycine gels (BioRad Mini-Protean TGX #4561094), and transferring onto nitrocellulose membranes using the BioRad Trans-Blot Turbo Transfer system. ..

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